Double Sucrose-Gap Method Applied to Single Muscle Fiber of Xenopus laevis

Double Sucrose-Gap Method Applied to Single Muscle Fiber of Xenopus laevis

Passive electrical properties (internal conductance, membrane conductance, low frequency capacity, and high frequency capacity obtained from the foot of the action potential) of normal and glycerol-treated muscle of Xenopus were determined with the intracellular microelectrode technique. The results show that the electrical properties of Xenopus muscle are essentially the same as those of frog ...

Hormone-sensitive stages in the sexual differentiation of laryngeal muscle fiber number in Xenopus laevis.

The number of muscle fibers in the vocal organ of the adult male African clawed frog, Xenopus laevis, exceeds that of adult females. This sex difference is the result of rapid fiber addition in males between the end of metamorphosis, post-metamorphic stage 0 (PM0) and PM2. At PM0, male and female frogs have similar numbers of laryngeal muscle fibers. Males then add more muscle fibers than femal...

ترتیب اعصای جانبی در دم لارو of Xenopus laevis

Androgen Regulation of Muscle Larynx of Xenopus laevis Type

We used histochemical techniques [assays for adenine triphosphatase (ATPase) and succinate dehydrogenase (SDHase) activity] to identify muscle fiber types in the larynx of Xenopus laewis. Male muscle is made up of one fiber type, medium-sized fibers (-9 e(m2) that stain lightly for acid-stable ATPase and intensely for SDHase activity. In contrast, the female has 3 fiber types: small fibers (-6 ...

Twitch and tetanic tension during culture of mature Xenopus laevis single muscle fibres.

Investigation of the mechanisms of muscle adaptation requires independent control of the regulating factors. The aim of the present study was to develop a serum-free medium to culture mature single muscle fibres of Xenopus laevis. As an example, we used the culture system to study adaptation of twitch and tetanic force characteristics, number of sarcomeres in series and fibre cross-section. Fib...